The introduction of biological preparations can be carried out in an intravenous, intraosseous, subcutaneous, intramuscular manner inside the affected organ. Most frequently it is administered by intravenous drop-by-drop introduction through a system of blood transfusion for 15-20 min. Besides the intravenous transfusion, cells can be introduced into tissues and organs. There is a certain tropism of cells to tissues of organs from which they originated. Thus, hepatic cells engrafted well upon implantation into the liver, nerve cells into neural tissue. Cardiac hystiocytes, cultured in vitro from their own stem cells, can be injected directly into the area of muscular tissue during heart operations.
The fact is that stem cells can accept any program and turn into any one of the possible types of embryonic cells; they are waiting for the signal to begin one of their transformations. Embryonic stem cells in contrast to all somatic cells do not have any functions except for the transfer of the mRNA into the next cells generation, it is a cartridge with information, the anonymous cell and as professor V. Repin has called it, the cell "without a name and patronymic".
At the same time, creation of a cells' depot in various tissues and organs is also allowed.
The whole procedure in accordance with the patient's state control takes one hour. With in the organism the introduced cells distributed non-uniformly. They possess "home instinct" and with in a few hours following injection, their content in hemopoietic organs is twenty times higher than anywhere else.
Notes:
Sometimes during the injection of biological preparations, patients feel overheated. These feelings pass quickly.
During the interphase stage (between two mitotic cell divisions) DNA present in the nucleus has molecule parts free from proteins which could block its activity. In these parts the transcription of a matrix of information RNA (mRNA) which through the nuclear interstices enter the cytoplasm occurs . Depending on the molecular structure and the cell's type, mRNA activity can take one of two possible routes:
The form of release
Ampules containing 0.5, 1.0, and 1.5 ml of cell suspension.
Storage conditions
Ampules are stored in liquid nitrogen at -196°C in special long-term storage until use. They are thawed up ex tempore in a water bath at +40° C.
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