Curriculum Vitae

 

Weiwen Deng, M.D., Ph.D.

Research Instructor

Department of Pharmacology, SL 83

Tulane University Health Sciences Center

1430 Tulane Avenue, New Orleans , LA 70112

Tel:  (504) 988 2762  

Fax: (504) 988 5283

E-mail: wdeng@tulane.edu

 

 

Specialties

 

 

 

 

Education

 

1990      M.D.   Shanghai Medical University, Shanghai, P.R. China

 

1993      M.S.    Shanghai Medical University

                          Major: Microbiology & Immunology

                          Thesis Title: In Vivo Transfection of Duck Hepatitis B Virus DNA

                          Mentor: Yumei Wen, M.D.

                                                                

2004      Ph.D.   Tulane University, New Orleans, LA, U.S.A

                          Major: Molecular & Cellular Biology

                          Dissertation Title: The Potential of Marrow Stromal Cells for Ex Vivo Gene

                                                        Therapy of Cardiovascular Diseases

                          Mentor: Philip J. Kadowitz, Ph.D.

 

                                      

Professional Experience and Appointments

 

1984.9-1990.7   Medical Student

                           Shanghai Medical University

 

1990.9-1991.1   Teaching Assistant

                           Department of Medical Microbiology,

                           Shanghai Medical University

 

1990.9-1993.7       Graduate Student

                           Microbiology & Immunology Program,

                           Laboratory of Medical Molecular Virology,

                           Shanghai Medical University

                           

1993.8-1994.12     Research Associate

Laboratory of Tumor Biotherapy,

                           Shanghai Institute of Cell Biology, Chinese Academy of Sciences,

                           Shanghai, P.R. China                        

 

1995.1-1996.1    International Centre for Scientific Culture-World Laboratory Training Fellow     

                           Laboratory of Tumor Immunotherapy,                            

                           Advanced Biotechnology Center, National Institute for Cancer Research,

                     Genoa, Italy

                         

1996.2-1997.7    Assistant Professor

                           Laboratory of Tumor Biotherapy,

                           Shanghai Institute of Cell Biology, Chinese Academy of Sciences

                           

1997.8-2000.6       Ph.D. candidate

                           Molecular Pathobiology Program,

Center for Gene Therapy,

Drexel University College of Medicine, Philadelphia, PA, U.S.A

Research projects:

                           “Neural differentiation of mesenchymal stem cells”;

                           “Therapeutic gene such as Fas ligand and IL-4 modified mesenchymal stem

                             cells for therapy of glioma”.

                         

2000.7-2004.4       Ph.D.

                           Molecular & Cellular Biology Program,

                           Center for Gene Therapy,

                           Department of Pharmacology,

                           Tulane University Health Sciences Center

                           Research project:

                            “The potential of mesenchymal stem cells for ex vivo gene therapy of

                              cardiovascular diseases”.

 

2004.5-2005.6   Postdoctoral Fellow

                           Department of Pharmacology,

                           Tulane University Health Sciences Center    

                           Research projects:

                             “Engineering ex vivo expanded mesenchymal stem cells with therapeutic gene

                               such as endothelial nitric oxide synthase, calcitonin gene-related peptide, and

                               superoxide dismutase”;

                             “Gene modified ex vivo expanded mesenchymal stem  cells for therapy of

                                erectile dysfunction in aged or diabetic rats”;

                             “Gene modified ex vivo expanded mesenchymal stem cells for therapy of

                                pulmonary hypertension in rats”.

                            Supervisor: Philip J. Kadowitz, Ph.D.

 

2005.7-present    Research Instructor             

                            Department of Pharmacology,

                            Tulane University Health Sciences Center

                            Research Topic:

                            Ex vivo gene therapy of erectile dysfunction and pulmonary hypertension using

                            marrow stromal cells

                            Supervisor: Philip J. Kadowitz, Ph.D.

 

            

Honor and Award:

 

1990        Shi & Li Fellowship, Shanghai Medical University

 

 

Membership

 

 

 

Research Support

 

Ongoing Research Support

     

 The Sexual Medicine Society of North America/Bayer 2004 Andrology Grant  

       “Gene modified ex vivo expanded marrow stromal cell transfer for gene therapy of

        erectile dysfunction in diabetic rats”.

        The project is to determine molecular alterations in diabetic erectile dysfunction (ED)

        and investigate the potential treatment of diabetic ED using marrow stromal cells

        containing target molecules, such as eNOS and nNOS.

        Role: Collaborator

 

             NIH/NHLBI

             “Stem cell therapy for pulmonary hypertension”.

              The broad long-term objectives of the proposed research are to develop a new

              mesenchymal stem-cell (MSC) based therapy for the treatment of pulmonary

              hypertension.

              Role: Postdoctoral Fellow and Co-Investigator

 

Completed Research Support

 

 NIH/NHLBI

 ”Cardiopulmonary Surgery Research”.

  The long-term objective of the research proposed in this application was to improve the

  current understanding of regulation of the pulmonary circulation by humoral factors,

  including vasoactive peptides.

  Role: Graduate Student         

 

 NIH/NHLBI

 ”Cardiopulmonary Surgery Research”

 The broad long-term objectives of the proposed research are to improve our current

 understanding of the regulation of the pulmonary vascular bed by humoral factors,

 including vasoactive products in the cyclooxygenase pathway.

             Role: Graduate Student

  

                                 

Patent

 
             United States Patent Application Serial No. 10/153,972 entitled “Directed In Vitro
             Differentiation of Marrow Stromal Cells into Neural Cell Progenitors” filed on May 23,
             2002 in the names of Darwin J. Prockop and Weiwen Deng  
 

     

Book Chapters

 

  1. Bivalacqua TJ, Deng W, Champion HC, Hellstrom WJ, Kadowitz PJ. Gene therapy techniques for the delivery of endothelial nitric oxide synthase to the corpora cavernosa for erectile dysfunction. In Aviv Hassid (ed.) Nitric Oxide Protocols, 2nd edition, pp173-186, Humana Press, Totowa, New Jersey, 2004 June

 

  1. Deng W, Baber SR, Bivalacqua TJ, Champion HC, Pradhan L, Hyman AL, Kadowitz PJ. Gene therapy techniques for the delivery of endothelial nitric oxide synthase to the lung for pulmonary hypertension. In Vincent F. LaRussa (ed.) Methods Mol Biol. Humana Press, Totowa, New Jersey, 2005, in press

 

 

Papers

 

  1. Deng W (1992). A model of creative thinking: the discovery of complement by Nobel laureate Dr. Jules Bordet. Medicine and Philosophy 8: 51-52

 

  1. Deng W (1993). The negative effect of recombinant DNA vaccines. Foreign Medicine × Microbiology Division 2: 49-50

 

  1. Deng W, Wen Y, He L, and Zhao S (1996). Study of in vivo transfection of duck hepatitis B virus (DHBV) DNA. Chinese Journal of Experimental and Clinical Virology 10: 23-26

 

  1. Deng W, Xie H, and Wen Y (1997). The research of in vivo transfection of cloned duck hepatitis B virus DNA dimer. Chinese Virology 12: 66-70

 

  1. Deng W, Xie H, Melioli G, Sergio B, Cangemi G, and Semino C (1997). Gamma ray-induced loss of expression of HLA-B8 antigen in melanoma cells. Chinese Journal of Immunology 13: 340-344

 

  1. Deng W, Xie H, Semino C, and Melioli G (1997). Rapid elution of tumor peptides from human lung caner cells by mild acid method. Shanghai Journal of Immunology 17: 37-40

 

  1. Semino C, Cilli M, Ratto GB, Deng W, Pietra G, Cangemi G, and Melioli G (1998). Limiting dilution analysis of peripheral blood lymphocytes reacting with non-small-cell lung cancer: functionally heterogeneous effectors efficiently lyse autologous cancer cells. Lung Cancer 21: 27-36

 

  1. Deng W, Obrocka M, Fischer I, and Prockop DJ (2001). In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Biochem Biophys Res Commun 282: 148-152

            (SCI citation: 90, by August 6, 2005)

 

  1. Deng W, Bivalacqua TJ, Chattergoon NN, Hyman AL, Jeter Jr JR, and Kadowitz PJ (2003). Adenoviral gene transfer of endothelial nitric oxide synthase: High level expression in ex vivo expanded marrow stromal cells. Am J Physiol Cell Physiol 285: C1322-1329

 

  1. Deng W, Bivalacqua TJ, Chattergoon NN, Jeter Jr JR, and Kadowitz PJ (2004). Engineering ex vivo expanded marrow stromal cells to secrete calcitonin gene-related peptide using adenoviral vector. Stem Cells 22: 1279-1291

 

  1. Chattergoon NN, D'Souza FM, Deng W, Chen H, Hyman AL, Kadowitz PJ, and Jeter Jr JR (2005). The anti-proliferative effects of calcitonin gene-related peptide in aortic and pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 288: L202-211

 

  1. Baber SR, Deng W, Rodriguez J, Master RG, Bivalacqua TJ, Hyman AL, and Kadowitz PJ (2005). Vasoactive prostanoids are generated from arachidonic acid by COX-1 and -2 in the mouse. Am J Physiol Heart Circ Physiol, 2005 Apr 29; [Epub ahead of print]

 

  1. Bunnell BA, Deng W, Robinson CM, Waldron PR, Bivalacqua TJ, Baber SR, Hyman AL, Kadowitz PJ (2005). Potential application for mesenchymal stem cells in the treatment of cardiovascular diseases. Can J Physiol Pharmacol 83: 529-539.

 

 

  1. Deng W, Bivalacqua TJ, Hellstrom WJG, Kadwotiz PJ. Gene and stem cell therapy for erectile dysfunction. Int J Impot Res, in press.

 

  1. Deng W, St. Hilaire RC, Chattergoon NN, Jeter Jr JR, Kadowitz PJ. Inhibition of vascular smooth muscle cell proliferation in vitro by genetically engineered marrow stromal cells secreting calcitonin gene-related peptide. (Submitted).

 

  1. Baber SR*, Deng W*, Bunnell BA, Taylor BK, Hyman AL, Kadowitz PJ. Intratracheal mesenchymal stem cell administration attenuates monocrotaline induced pulmonary hypertension and endothelial dysfunction.  *: equal contributors. (Submitted)

 

  1. Mesenchymal stem cells alone or modified with eNOS restore erectile function in the aged rat: cell-based gene therapy for erectile dysfunction. (In preparation)

 

  1. Gene therapy of pulmonary hypertension in rats using marrow stromal cells genetically engineered to secrete calcitonin-gene related peptide. (In preparation)

 

  1. Adenoviral gene transfer of superoxide dismutase: high level expression in ex vivo expanded marrow stromal cells. (In preparation)

 

 

Abstracts

 

  1. Deng W, Obrocka M, Fischer I, and Prockop DJ. In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Mesenchymal and nonhematopoietic stem cells: recent progress and current controversies, March 22-24, 2001, New Orleans, LA.

 

  1. Deng W, St. Hilaire RC, Chattergoon NN, Jeter Jr JR, and Kadowitz PJ. Inhibition of vascular smooth muscle cell proliferation in vitro by genetically engineered marrow stromal cells secreting calcitonin gene-related peptide. ISHR-International Society for Heart Research American Section 27th Annual Meeting, May 12-15, 2005, New Orleans, LA.